![]() ![]() Reports showed that as of June 16, 2020, at least 440,421 confirmed deaths and more than 8,144,359 confirmed cases, and the current statistics as of November 27 are currently at 1.4 million deaths with 61.2 million cases of infection. It was later termed SARS-CoV-2 and the disease COVID-19 by the WHO. On December 31, 2019, the disease outbreak was traced to Coronavirus’s novel strain. There was a rapid spread of the disease from the city of Wuhan to many countries, including the United States, with thousands infected and many dying within months of initial spread. The physiochemical properties of the protein sequence such as the GRAVY (Grand average of hydropathicity), half-life, molecular weight, instability index, aliphatic index, and amino acid atomic composition was bio-computed via an online tool Protparam ( ).įunding: The authors did not receive any specific grant or funds from any funding agencies in the public, commercial, or not-for-profit sectors, during the research and manuscript preparations.Ĭompeting interests: The authors have declared that no competing interests exist.Īn unprecedented pneumonia disease outbreak was reported in late December 2019, after several deaths were recorded in Wuhan, China. The protein structure of the SARS-CoV-2 spike (PDB: 6VSB) was downloaded from the protein data bank. The protein sequences from different geographical regions were retrieved from the NCBI repository with their corresponding accession numbers: Wuhan, China (Genbank ID: QHD43416.1), Japan (Genbank ID: BCA87361.1), California, USA (Genbank ID: QHQ71963.1), Washington, USA (Genbank ID: QHO60594.1), and Valencia, Spain (Genbank ID: QIQ08790.1). This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.ĭata Availability: All relevant data are within the manuscript and its Supporting Information files. Received: JAccepted: FebruPublished: March 17, 2021Ĭopyright: © 2021 Chukwudozie et al. PLoS ONE 16(3):Įditor: Pinyi Lu, Frederick National Laboratory for Cancer Research, UNITED STATES (2021) Immuno-informatics design of a multimeric epitope peptide based vaccine targeting SARS-CoV-2 spike glycoprotein. We, therefore, propose that potential vaccine designs consider this approach.Ĭitation: Chukwudozie OS, Gray CM, Fagbayi TA, Chukwuanukwu RC, Oyebanji VO, Bankole TT, et al. Upon in silico challenge with SARS-CoV-2, there was a decrease in antigen levels using our immune simulations. The vaccine was also characterized by an increase in IgM and IgG and an array of Th1 and Th2 cytokines. With our In silico test, the vaccine construct was predicted to elicit high antigenicity and cell-mediated immunity when given as a homologous prime-boost, triggering of toll-like receptor 5 by the adjuvant linker. We used this information to construct an in silico chimeric peptide vaccine whose translational rate was highly expressed when cloned in pET28a (+) vector. We identified eight CD8+ T cell 9-mers and 12 CD4+ T cell 14-15-mer as promising candidate epitopes putatively restricted by a large number of MHC I and II alleles, respectively. Five antigenic B cell epitopes with viable antigenicity and a total of 27 discontinuous B cell epitopes were mapped out structurally in the spike protein for antibody recognition. We have used a bioinformatic approach to aid in designing an epitope peptide-based vaccine against the spike protein of the virus. Developing an efficacious vaccine for SARS-CoV-2 infection is critical to stemming COVID-19 fatalities and providing the global community with immune protection. ![]()
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Media rooms are painted dark to minimize light reflections and allow the media screen to pop out from the contrasting backdrop. ![]() You can match with SW 7070 Site White, SW 7074 Software, and SW 7012 Creamy as your accents. C hoose a matte or eggshell finish to prevent reflections while still achieving a sober atmosphere for your home theater. LRV (Light Reflectance Value) – 5.89%: The pale red is a perfect home theater paint if you’re looking for a more traditional and livelier color tone. The best strategy is to have a well-thought lighting design to prevent glare and other viewing issues from achieving a mood. T he deep dark red can exude a feeling of luxury for any home theater but can be challenging when it comes to lighting and combining them with other colors. LRV (Light Reflectance Value) – 6.7%: Burgundy is a familiar hue that most of us associate with the traditional movie house and theater. ![]() Still, unlike black, it is not visually overwhelming, making it possible to match with other colors and apply to a larger area. Like black, gray is a neutral hue that’s excellent in pairing with other colors. ![]() LRV (Light Reflectance Value) – 8%: Another less distracting paint option for your home theater is a color under the gray range. The Black Fox SW 7020 is also great for small home theaters as it opens up the space with its lighter tone. LRV (Light Reflectance Value) – 8%: The Black Fox SW 7020 boasts a rich brown undertone that’s perfect for homeowners looking for a paint color for their home theater that leans more towards a warmer hue.Īnother great thing about the medium-dark tone is that it still gives a modern vibe while retaining a cozy atmosphere. In addition, the deep black is an excellent contrast and a stunning color that makes your focal point (your media screen) stand out. LRV (Light Reflectance Value) – 2.8-3%: If you want to have a home theater paint color that goes with other colors of your furniture, carpet, and ceiling, the Tricorn Black SW 6258 is a safe choice since it has no visible undertones. A close alternative to Federal Standard 27038 is Farrow & Ball Off-Black 57, which has the same dramatic and easy-on-the-eyes quality. It works with most home theater dimensions, especially for small areas, as it keeps to room feeling open and relatively feeling light. The Federal Standard 27038 is close to dark gray shades with a tinge of blue and green colors. LRV (Light Reflectance Value) – 2.6%: With a low LRV rating, this is an excellent choice for any home theater design. If you want to add a warmer touch to your blue theater, choose velvet or leather brown reclining seats to balance the look. It breaks away from the traditional colors black and burgundy of commercial theaters but makes sure to choose the darkest shade possible.Īnchors Aweigh SW 9179 is another darker nautical shade that matches great with white or brown furniture. LRV (Light Reflectance Value) – 3.02%: Nautical colors are a popular choice for home theaters. LRV (Light Reflectance Value) – 4%: Whether you have a small or large home theater, this cool dark blue, green tinge is an excellent choice for a modern and simple home theater with a naval theme.Įither paint your home theater purely with the Dark Night SW 6237 color or combine it with accent colors such as Warm Stone SW 7032 and Peppercorn SW 7674. Here we share the different paint ideas for home theaters. Meanwhile, gray and black are among the home theater wall colors that give off a cooler, more sophisticated modern look. Warmer colors such as dark brown or burgundy make a room cozier. When choosing colors, consider the style and theme you are going for, as room color equates to the atmosphere. These dark colors contrast the projected screen, allowing viewers to see the images clearly and preventing glare and sheen from light bouncing to and from surfaces. The best paint color for a home theater is generally dark matte colors ranging from brown-gray to black with strategic lighting. What Is The Best Background Color For TV?. ![]() ![]() But in some places, you have the right to refuse and even sue.Īs the collection and use of biometrics has exploded, lawmakers in three states have responded by passing laws restricting its deployment. Privacy and labor laws have lagged behind the shifts in the American workplace. Other companies have not responded to requests for comment or have said they cannot comment on active litigation. In some cases, the companies have countered in the lawsuits that their employees’ union agreement allows the use of the machines: “Southwest and United contend that the plaintiffs’ unions have consented-either expressly or through the collective bargaining agreements’ management-rights clauses-and that any required notice has been provided to the unions,” the court’s opinion states. “You can get a new phone, you can change your password, you can even change your Social Security number you can’t change your face,” said Kade Crockford, the Technology for Liberty program director at ACLU of Massachusetts.Ĭompanies facing legal action over their use of the machines range from fast food joints like McDonald’s and Wendy’s, to hotel chains like Marriott and Hyatt, to airlines like United and Southwest. In an amicus brief in a case before a federal court of appeals, a group of privacy advocates, including the ACLU and the EFF, wrote that “the immutability of biometric information” puts people “at risk of irreparable harm in the form of identity theft and/or tracking.” In addition to these health concerns, critics argue that biometric devices pose massive personal security issues, exposing workers to potential identity theft and subjecting them to possible surveillance from corporations and law enforcement. And you should wash your hands for 20 seconds or use alcohol-based hand sanitizer immediately after using one. ![]() The Centers for Disease Control says that coronavirus can remain on surfaces for hours, so screens and scanners should be regularly disinfected with cleaning spray or wipes. With the global o utbreak of COVID-19, your personal health could be at risk when using frequently touched screens and fingerprint scanners. ![]() Because it turns out moving fast and breaking things broke some super important things. ![]() ![]() The Fire HD 10 2021 (left) and the iPad (right) (Image credit: Michael Hicks / Future)Īmazon claims the Fire HD 10 (2021) got a 10% bump in brightness (nits) from the 2019 model. Holding it in one hand unsupported while swiping with the other for a prolonged stretch isn't particularly comfortable. Like most tablets of this size, we found it most convenient to hold with two hands and the bottom propped against our stomach, or set on a flat surface with a kickstand. The Plus version only ships in slate gray, with a softer finish that you may find more comfortable to hold. ![]() You can choose between one of four colors: black, denim (dark blue), lavender (pinkish) or olive (green). You do need to watch out for spills, however, as the Amazon Fire HD 10 (2021) has no reported water resistance. With a plastic back, the Fire tablet doesn't have the snazzy metal finish you see on some other devices, but at least you don't need to worry as much about scuffs or falls. Measuring 247 x 166 x 9.2mm with a 10.1-inch LCD display, the Fire HD 10 (2021) has thick bezels on the top and bottom, giving it a lower screen-to-body ratio than recent tablets with edge-to-edge displays. Otherwise, most of the differences between them are internal these Fire tablets still aren't winning any beauty contests. 504g) with a slightly more compact frame. The Amazon Fire HD 10 (2021) received a slight redesign compared to the 2019 model, weighing slightly less (465g vs.
![]() ![]() ![]() Kinda like Red Knight's stunlocking splash magic. Giving up splash magic to heal between waves is a crappy player's best friend. ![]() On the other hand I wouldn't throw King on that list. Why isn't Alien on the list? He loses out on a pretty big boost at endgame (though I guess he's fine until then.), and while his magic isn't bad I wouldn't call it some kind of great equalizer. Melee is king though, so I'd be more prone to throw Alien (Stuck with starter weapon) or Green Knight (Forced to not juggle or use crappier juggles) under the bus before him. Originally posted by Ittrix:I mean in terms of magic blacksmith's pretty bad. And magic is what clutches insane mode, all the top players alike have a roughly similar list of characters they hate using because they don't get any utility out of their magic, and blacksmith is the one who everyone agrees is the worst. Every character can reach max level though. He's meant to be a melee build, of course his magic is terrible. Are you seriously judging the Blacksmith, exclusively by his magic? He's still undeniably a bad character though. Note: I totally understand using blacksmith if you think he's cool. Not to be confused with Barbarian who is actually quite decent.) What's that? His projectile is good? Just use Fire Demon instead, he has the same projectile only he has the second best splash attack in the game.īottom five characters (basically honorable mentions)ĥ) Arrow Rainers (Thief Clones. It's a waste for him to use it in the air. His splash has a long startup, and it has no knockback. Originally posted by 3 Damage to Any Target:It's Blacksmith. ![]() ![]() It not only showed the beauty of the country of which we reside, but it also spoke about the people that reside in it. This film should be shown in every American History class in the United States. Oddly, this did occur, but this film was much more than that. I was expecting nothing more than some short scenes of our now-infamous actors smoking marijuana followed by trippy Willy Wonka scenes. On the whole, this film is an out and out classic. Classic rock accompanies the pictures of the two men rider their bikes, and it's very cool indeed. Music was, of course, a big thing in the sixties and it's a big thing about this movie. Last but not least, another great thing about Easy Rider is the music. Even here, he shows his charisma and ability to steal the show and that is what he would go on to become famous for doing later in his career. It's not hard to see why this actor went on to become one of the best of all time. Jack Nicholson turns up halfway through and steals the show. The two actors brilliantly get into their characters, and after a while you forget that you're watching actors and start to think that these people really are these characters. The acting performances are a big part of the movie, and the two leads Peter Fonda and Dennis Hopper stick out the most. That's the theme of the entire movie, and the way that it plays out, and the ending especially, aptly portray the difference between saying something and actually doing it. But like George Hanson says it's one thing to talk about being free, but something else entirely to actually be it. The American Dream has always been about freedom. We follow their exploits as they travel the country meeting various people including, most notably, George Hanson an offbeat lawyer, played by the great Jack Nicholson. ![]() It follows two motorbike riders on their way from Los Angeles to the Mardi Gras in New Orleans. The plot is simple and more just a base for the film to deliver it's real sting than anything else. These two were obviously in the thick of what was cool in the sixties, and that gives the film an element of authenticity as we feel like what we're seeing isn't too far away from the things really going on at that time. Dennis Hopper and Peter Fonda, men of substance and substance abuse, wrote the film together and Hopper directed it. This film is very much a product of the sixties and, like many things from the decade, will always be fondly remembered. Dennis Hopper's Easy Rider is often cited as being an all time classic, and while I don't think this is a great film in terms of technical brilliance, it sums up the era it was made and the tongue in cheek, cynical take on the 'American dream' is both potent and well done. ![]() |
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